USE OF PROTEIN ELECTROPHORESIS FOR QUALITATIVE AND QUANTITATIVE DIFFERENTIATION OF POWDERY MILDEW (Blumeria graminis) DISEASE SEVERITY ON BARLEY GENOTYPES
نویسندگان
چکیده
منابع مشابه
DNA polymorphism among barley NILs of cv. Pallas, carrying genes for resistance to powdery mildew (Blumeria graminis f. sp. hordei).
Barley powdery mildew, caused by the pathogen Blumeria graminis f. sp. hordei is an important disease of barley (Hordeum vulgare L.). The random amplified polymorphic DNA (RAPD) method was used to detect DNA polymorphism among 7 Pallas near-isogenic lines (NILs) carrying Mla3, Mla12, Mlk, Mlp, Mlat, Mlg and MlLa genes for resistance to B. graminis f. sp. hordei. From among 500 random 10-mer pri...
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Reactive oxygen intermediates (ROI) are closely related to defence reactions of plants against pathogens. A prominent role in the production of ROI has been attributed to the plant respiratory burst oxidase homologues (RBOH) of the human phagocyte GP91(phox). A barley RBOH, which encodes a putative superoxide (O2*-)) producing NADPH oxidase, is described here. Histochemical analysis of the barl...
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The activity of esterase secreted by conidia of wheat powdery mildew fungus, Blumeria graminis f. sp. tritici, was assayed using indoxyl acetate hydrolysis, which generates indigo blue crystals. Mature, ungerminated, and germinating conidia secrete esterase(s) on artificial media and on plant leaf surfaces. The activity of these esterases was inhibited by diisopropyl fluorophosphate, which is s...
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Barley is one of the most important cereals in animal nutrition and it has a role as a substitute source of wheat and rice in diet nutrition. This product is always exposed to damaging factors such as pests and diseases which lead to reduce yield and quality. The Powdery mildew agent, as a biotrophic fungus, seriously causes damage in some barley plantation areas. Similar to other plants, barle...
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Powdery millews usually grow superficially on their hosts but produce symptoms of disease throughout the underlying host tissues. The symptoms include marked increase in respiratory rate (1, 3, 15. 24, 26, 30. 35); accumulation of organic and inorganic substances (1,29, 36): increase in dry weight (10. 26) ; increase in wet weight (3) decrease in photosynthesis when the disease is well develope...
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ژورنال
عنوان ژورنال: Journal of Plant Protection and Pathology
سال: 2016
ISSN: 2090-3758
DOI: 10.21608/jppp.2016.50056